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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
02/03/2017 |
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Actualizado : |
07/03/2017 |
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Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
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Idioma : |
Inglés |
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Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02512naa a2200217 a 4500
001 1056750
005 2017-03-07
008 2015 bl uuuu u00u1 u #d
100 1 $aGARCIA, J.P
245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource]
260 $c2015
520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease.
650 $aENFERMEDADES DE LOS ANIMALES
700 1 $aGIANNITTI, F.
700 1 $aFINNIE, J.W.
700 1 $aMANAVIS, J. .
700 1 $aBEINGESSER, J.
700 1 $aADAMS ,V.
700 1 $aROOD, J.I.
700 1 $aUZAL, F.A.
773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
22/02/2014 |
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Autor : |
Arboleya, J. |
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Título : |
Proyecto: Manejo y fisiología de cultivo y de la poscosecha HO_05 |
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Fecha de publicación : |
2007 |
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Fuente / Imprenta : |
Las Brujas, Canelones (Uruguay): INIA, 2007. |
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Páginas : |
p.12-13 |
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Serie : |
(INIA Serie Actividades de Difusión ; 479) |
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Idioma : |
Español |
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Notas : |
INIA Las Brujas |
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Thesagro : |
CULTIVOS; ESCARDA; SOLARIZACION DE SUELOS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/492/1/111219220807115946.pdf
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Marc : |
LEADER 00520naa a2200181 a 4500
001 1008661
005 2014-02-22
008 2007 bl uuuu u00u1 u #d
100 1 $aARBOLEYA, J.
245 $aProyecto$bManejo y fisiología de cultivo y de la poscosecha HO_05
260 $c2007
300 $ap.12-13
490 $a(INIA Serie Actividades de Difusión ; 479)
500 $aINIA Las Brujas
650 $aCULTIVOS
650 $aESCARDA
650 $aSOLARIZACION DE SUELOS
773 $tLas Brujas, Canelones (Uruguay): INIA, 2007.
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